Group members:
Ph.D. Obluchinskaya Ekaterina Dmitrievna - head of the group
Dr., Prof. Shikov Alexander Nikolaevich
Ph.D. Pozharitskaya Olga Nikolaevna
junior researcher Daurtseva Anna Vasilievna
leading engineer Gorshenina Elena Vyacheslavovna
Since 2017, the research group has become part of the zoobenthos laboratory.
Main areas of work:
- analysis and production of biologically active substances (BAS) of algae and invertebrates, their biopharmaceutical evaluation;
- study of the qualitative and quantitative characteristics of BAS of hydrobionts (polysaccharides, polyphenols, pigments, proteins, amino acids, enzymes, etc.);
- study of the effect of biotic and abiotic factors on the composition and content of BAS;
- development of innovative technologies for the production of BAS in seaweeds and invertebrates;
- obtaining functional products and bioproducts with therapeutic and prophylactic properties, the study of their pharmacological activity.
Main achievements:
- the concept of rational use of algal resources is proposed;
- an original method for obtaining the active pharmaceutical substance fucoidan, which has an anticoagulant effect, has been developed (RF Patent No. 2506089, 2014);
- the new substance fucoidan has passed a full cycle of preclinical studies with the financial support of the Federal Target Program "Development of the Pharmaceutical and Medical Industry of the Russian Federation for the period up to 2020 and beyond", based on it, drugs have been created for the treatment of thrombosis and thrombophlebitis of the veins of the lower extremities, which are recommended for clinical trials;
- for the first time, deep natural eutectic solvents have been proposed for the extraction of biologically active substances from brown algae as an alternative to organic solvents and the implementation of the principles of "green" technologies.
Main publications 2017-2021:
01. Obluchinskaya, E. D., Pozharitskaya, O. N., Flisyuk, E. V., & Shikov, A. N. (2021). Formulation, Optimization and In Vivo Evaluation of Fucoidan-Based Cream with Anti-Inflammatory Properties. Marine Drugs, 19(11), 643. https://doi.org/10.3390/md19110643
02. Obluchinskaya, E.D., Pozharitskaya, O.N., Zakharova, L.V., Daurtseva, A.V., Flisyuk, E.V. and Shikov, A.N., 2021. Efficacy of Natural Deep Eutectic Solvents for Extraction of Hydrophilic and Lipophilic Compounds from Fucus vesiculosus. Molecules, 26(14), p.4198. https://doi.org/10.3390/molecules26144198
03. Ayrapetyan, O.N.; Obluchinskaya, E.D.; Zhurishkina, E.V.; Skorik, Y.A.; Lebedev, D.V.; Kulminskaya, A.A.; Lapina, I.M. Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea. Biology 2021, 10(1):67. https://www.mdpi.com/2079-7737/10/1/67/htm
04. Obluchinskaya, E.D., Zakharova, L.V. Comparative study of polyphenols of brown algae of the Barents sea and the White sea, as well as the waters of the North Atlantic // Khimiya Rastitel'nogo Syr'ya, 2021 Is. 4, P. 129-137= Obluchinskaya E.D., Zakharova L.V. "Comparative study of brown algae polyphenols from the seas of the Arctic and North Atlantic" // Chemistry of plant raw materials - 2020- No. 4 - P. 129-137 DOI: 10.14258/jcprm.2020047755 (Scopus)
05. Obluchinskaya E., Zakharova L. Metal concentrations in three species of Fucus L. on the Murmansk coast of the Barents Sea // Polar Science. - 2021. - S. 100646.
06. Derkach S.R., Kolotova D.S., Voron'ko N.G., Obluchinskaya E.D., Malkin A.Y. Rheological Properties of Fish Gelatin Modified with Sodium Alginate. polymers. 2021; 13(5):743. https://doi.org/10.3390/polym13050743
07. Obluchinskaya E.D. Effect of different post-harvest treatments on free amino acid content in Fucus vesiculosus //KnE Life Sciences. - 2020. - S. 386-395-386-395.
08. Obluchinskaya E.D, Daurtseva A.V. Effects of air drying and freezing and long-term storage on phytochemical composition of brown seaweeds // Journal of Applied Phycology. - 2020. - V. 32 - P. 4235-4249 https://doi.org/10.1007/s10811-020-02225-x
09. Obluchinskaya E.D., Pozharitskaya O.N., Flisyuk E.V., Shikov A.N. Optimization of the composition and technology for obtaining tablets with fucoidan and their biopharmaceutical evaluation // Chemical Pharmaceutical Journal. - 2020. - T. 54. - No. 5. - S. 38-42. https://doi.org/10.30906/0023-1134-2020-54-5-38-42= Obluchinskaya E.D., Pozharitskaya, O.N., Flisyuk, E.V., Shikov, A.N. Optimization of the Composition and Production Technology of Fucoidan Tablets and their Biopharmaceutical Evaluation //Pharmaceutical Chemistry Journal. 2020. P. 1-5 https://doi.org/10.1007/s11094-020-02237-4
10. Pozharitskaya O. N., Obluchinskaya E. D., Shikov A. N. Mechanisms of Bioactivities of Fucoidan from the Brown Seaweed Fucus vesiculosus L. of the Barents Sea //Marine Drugs. - 2020. - T. 18. - No. 5. - P. 275. https://doi.org/10.3390/md18050275
11. Shikov, A.N.; Flisyuk, E.V.; Obluchinskaya, E.D.; Pozharitskaya, O.N. Pharmacokinetics of Marine Derived Drugs. Mar. Drugs 2020, 18, 557. https://doi.org/10.3390/md18110557
12. Obluchinskaya, E.D., Daurtseva, A.V., Pozharitskaya, O.N., Flisyuk, E.V., Shikov, A.N. Natural deep eutectic solvents as alternative extractants for the extraction of brown algae phlorotannins / / Chem.-farm. journal, vol. 53. No. 3. 2019. p. 30-34. = Obluchinskaya E.D., Daurtseva A.V., Pozharitskaya O.N., Flisyuk E.V., Shikov A.N. Natural deep eutectic solvents as alternatives for extracting phlorotannins from brown algae // Pharmaceutical Chemistry Journal, Vol. 53, no. 3, June, 2019. P. 243-247 (Russian Original Vol. 53, No. 3, March, 2019)
13. Pozharitskaya, O.N., Shikov, A.N., Obluchinskaya, E.D., Vuorela, H. The pharmacokinetics of fucoidan after topical application to rats// Marine drugs.- 2019.-17(12),- 687.
14. Voron'ko, NG, Derkach, SR, Kuchina, YA, Sokolan, NI, Kuranova, LK, Obluchinskaya, ED, Influence of added gelatin on the rheological properties of a Fucus vesiculosus extract, Food Bioscience 29 (2019) 1 -eight.
15. Pozharitskaya, O.N., Shikov, A.N., Faustova, N.M., Obluchinskaya, E.D., Kosman, V.M., Makarov, V.G. Pharmacokinetic and Tissue Disposition of Fucoidan from Fucus vesiculosus after Oral Administration to Rats // Mar. Drugs 2018, 16(4), 132.
16. Makarevich, P.R., Obluchinskaya, E.D., Dvoretsky, A.G., Zhuravleva, N.G. Modern trends in the breeding and cultivation of non-traditional aquaculture objects (Arctic char, king crab, sea urchin) and technologies for processing hydrobionts / P.R. Makarevich // Bulletin of MSTU. - 2018. - Volume 21, No. 2. - S. 355-370.
17. Kosman V.M., Obluchinskaya E.D., Pozharitskaya O.N., Makarova M.N., Shikov A.N. End-to-end standardization of the fucoidan substance from Fucus vesiculosus L. and preparations based on it // Pharmacy, 2017, v. 66. No. 6, p. 20-24.
Patents:
- "Dry extract of fucus, method of its preparation and anticoagulant ointment based on it". RF Patent No. 2506089 // B.I. No. 4, 10.02.2014,
- "Dry extract from fucus algae with antioxidant activity, and method for its production". RF patent No. 2650808// B.I. No. 11, 04/17/2018,
- "Pharmaceutical composition based on fucoidan for oral use and method for its preparation". RF Patent No. 2657615// B.I. No. 17, 06/14/2018,
- "Method for obtaining enzymatic hydrolysates from marine hydrobiont processing wastes". RF Patent No. 2712747 // B.I. No. 4, 01/30/2020
MMBI RAS integrated technology for the processing of fucus algae
